The second frozen electro -mirror -conducting electric hair structure is actually cytochrome?

Author:Bioart biological art Time:2022.07.10

Written article 丨 Dr. Wang Feng Bin

"TRUTH NEVER TRIUMPHS -ITS OPPONENTS Just Die Out." -Max Planck. Planck's meaning is probably "Old Theories Never Die; Some scientific research areas do have some embarrassing phenomena. One direction has stagnated because the big guy in the field many years ago brought others to the pit, and then the big man was unknown. Do not admit life. What I want to tell today is such a story (Editor's Note: On July 7, 2022, Dr. Feng Bin, the University of Virginia, published an article in the Nature Microbiology as the first author. Packing).

Derrick Old Iron is a well -known microbiologist. A kind of anaerobic bacteria was found in the river sedimentation of Washington DC 35 years ago. This bacteria is great. It can produce a few micrometers of "conductive hair" that conducts electronics at a long distance and perform energy metabolism. Derek's research on this conductive hair is 30 years. Later, they discovered that once they knocked down a "fourth type of bacteria" named Pila-N, the conductive hair was gone. Pila-n, the structure is just a very hydrophobic Helix, which is the core of hydrophobic in the middle of the fourth type of bacteria. Although Pila-N is a problem in the eyes of many structural biologists, it is a problem that the Derrick Old Iron has determined that the conductive hair must be Pila-N, and firmly believes that he can keep the clouds and see the moon.

With the technological revolution of freezing electron microscope, now everyone does not have to rely on genetic experiments to make these judgments every day. Want to know what the electrical wool is? Put it under the freeze electron microscope. In 2019, we directly observed the conductive hair with frozen electron microscopy. As for its relationship with the fourth type of fungal puff protein, it can only be said to be unrelated. The conductive hair is actually a Multi-HEME Cytochrome formed a bacterial hair that has never been discovered before, and the cytossence of Multi-Heme, everyone knows that they can transmit electronics (see Bioart for details: Cell | Dr. Wang Feng Bin, etc. Analyze the frozen electron microscopy structure of the bacterial conductive nanivor).

The deeric Iron did not read this reality, but continued to choose the fourth type of bacteria. His hissing questioned, why did he change Pila-N, and the conductive hair was gone? ah? Niqiu Old Iron was a former postdoctoral of Derrick. He is now a professor of a prestigious school. A relatively convincing model was published in 2021, saying that the fourth type of bacteria included two protein Pila-N and Pila-C in the bacteria. It is equivalent to a pump. If there is nothing to move, the conductive hair formed by cytochrome is gone out. (Ref: https://doi.org/10.1038/s41586-021-03857-w) Derrick's old iron was completely angry, saying "freezing electron microscope can not see the 3nm Pila-N" conductive hair ". It means that it does not exist! I can see it with AFM! You frozen electron microscopes are allifact! "You see, this is not a coincidence. We have recently made some other frozen electron microscope observations. We have mutated the key amino acids of the initial "conductive hair", and we wanted to study the first -generation conductive hair, which originally wanted to study mutation. You guess what to do. If you use a general Promoter to express mutation, we can't see the first -generation conductive hair of mutation at all, but we see a new conductive hair, OMCE. Guess what he is, or cytochrome. Who can think of the cells so "smart", even the alternatives of the first -generation electrical wool are quietly saved. If you use a promoter, you can not only see OMCE, but also some bundles of the first generation of bacteria, as well as a small number Pila-n is likely to be irreplaceable). It may be that the expression is too violent, and the pump is too fierce, and I have stabbed myself.

Figure OMCS A substitute for conductive hair, OMCE

So, there is really no 3nm hair? Is it so bad for Derrick's old iron eyes? In fact, there is really a hair of about 2.5nm, which occasionally appears. The DNASE I will disappear, yes, it is-B-Form DNA.