Zhang Xianghan and Wang Zhongliang team of Xi'an Electronic Science and Technology University Student Science

Recently, Professor Zhang Xianghan and Professor Wang Zhongliang of the School of Life Science and Technology of Xi'an University of Electronic Science and Technology collaborated with Professor Pu Kan of Nanyang University of Science and Technology in Singapore to work in the comprehensive sub -category of NatureCommunication (NatureCommunications "in the international comprehensive category. IF = 14.919), published a research paper entitled "BioorthogonallyActivityANEDYEWITORSION-InDucedDisaggregationforimaging" (Doi: 10.1038/S41467-022-31136-3). The first unit of thesis is Xi'an University of Electronic Science and Technology. Associate Professor Zhang Xianghan at the School of Life Sciences and Technology is the first author of the paper. Professor Wang Zhongliang, Professor Tian Jie, and Professor Pu Kan of Nanyang University of Science and Technology in Singapore as the common communication authors of the paper.

In recent years, nearly infrared fluorescent imaging technology has played a very important role in the early tumor diagnosis, accurate navigation of optical surgery, and real -time visualization effect assessment. In particular, the smart response type near -infrared fluorescent probe is a key technology for improving imaging specificity and sensitivity. one. Biological orthodontic chemistry has the advantages of high selectivity, rapid response and good biocompatibility. It can be visually visualized in the dynamic process of internal molecules, pre -targeted imaging of disease logo, in -situ metabolic signs and treatment Extensive application, and biological orthogonal technology has begun to conduct trials in clinical practice. Among all biological orthogonal chemical modules, Tetrazine (TZ) biological orthogonal probes not only have targeted functions, but also activate the target The signal, realize the "turn-on" or amplification of the fluorescent signal to achieve the effect of "targeted activation dual function" (2-in-1).

However, the vast majority of biological orthogonal activation probes are concentrated in visible light areas. There are very few probes near the infrared zone. It is mainly limited by TZ's quenching mechanism (TBET, PET, FRET, ICT). Application in the field of biomedical. Therefore, designing and constructing smart biological orthodox near infrared fluorescent probes is still a very challenging job. Based on the early work (Theranostics2017,7 (15): 3794-3802; nanoresearch2021,14,2432-2440), the author proposed a new Torsion-InducedDisaggregation, Tida) biological positive positive signal amplification amplification amplification signal amplification amplification signal amplification amplification amplification amplification amplification amplification amplification amplification amplification amplification signal amplification amplification amplifier amplification amplification amplifier amplification amplifier amplification amplification amplification amplification amplification amplification amplification amplification signal amplification amplification amplification amplification New mechanism. This mechanism is not restricted by the tz quenching mechanism. The spatial blocking effect of the molecular orthodontic response is used to increase the spatial resistance effect of the tetrazine biological orthogonal reaction to achieve the activation of fluorescent signals.

According to the analysis of the nuclear magnetic one -dimensional/two -dimensional hydrogen spectrum, quantum chemistry, and molecular dynamics, it is found that unlike the previous TZ hamstormation mechanism, the near -infrared fluorescent probe designed by this thesis will occur through the biological orthodontic response, and the molecular configuration will occur by S. -Trans to the molecular from the molecular from S-CIS, which increases the molecular space resistance, and triggers the phenomenon of TIDA fluorescence. Secondly, most of the organic dyes have the problem of the fluorescent quenching (ACQ) in the molecule. For example, the fluorescent quenching of the near -infrared fluorescent probe approved by the FDA approved clinical use can reach more than 80%(≥4 μm), which is serious. It affects the imaging sensitivity of ICG. The TIDA-type fluorescent molecules designed in this thesis have increased the accumulation of π-π in the co-scalp system by reversing the angle, causing the fluorescent group to solve the agglomeration (almost no fluorescence loss) ACQ problem.

Further, the near -infrared intelligent response probe based on TIDA -based construction of the infrared intelligent response to the cell level achieved high -specific biological orthogonal activation imaging; 5 minutes after the tail intravenous injection of lotus tumor mice, the TZ intelligent response probe can light up the tumor, and the tumor can be on. Realize high -sensitive imaging testing of tiny tumors. Therefore, this work proposes a new TIDA design mechanism that builds biological orthogonal to activate the NIR fluorescent group, and has opened up new ideas for the use of biological orthogonal chemistry in the body imaging, which has theoretical and applied significance.

It is reported that Professor Wang Zhongliang and Associate Professor Zhang Xianghan have been engaged in the construction of near -infrared fluorescent probes for many years and research on biomedical application. They have published important research results in domestic and foreign journals, including nat.commun., Angew.chem., Adv.mater., Adv.funct.Mater., ACSNANO, Sci.Bull., Theranostics, ACSAPPL.MATER.INTERFACES, Nanores. Experts quoted and positively evaluated. (Correspondent: Rao Zhiping, Xi'an University of Electronic Science and Technology)

- END -