"Broken hands and feet" can also be renewed!The relevant research results of this university appeared on the "Science" subsidiary

"Broken hands and feet" can be renewed? This is not from the plot in film and television dramas or science fiction movies. Recently, the team of Professor Wang Chenghui of the School of Aquatic and Life of Shanghai Ocean University, Professor Li Chenhong, Joint College, Professor Michael Hofreiter of the University of Potsdam, Germany, Professor Lu Guoqing, the University of Nebraska University in the United States, and other researchers. Regeneration research has made progress. Related research results were published in "Science Advances".

Professor Wang Chenghui, Professor Li Chenhong, and Professor Michael Hofreiter of the University of Potsdam, Germany, as the author of the paper, associate Professor Wang Jun and Dr. Chen Xiaowen of Shanghai Ocean University as the co -first author. Studies have been funded by Shanghai Science and Technology Promotional Agricultural Key Research Project and Shanghai Chinese Classy Crab Modern Agricultural Industry Technology System Project.

High -quality completion of the sequence and assembly of the whole genome of Chinese velvet crab

Chinese velvet crab is the most common crab variety on the Chinese dining table, and it is also my country's high economic value of aquatic crustaceans. There are 28 provinces and cities in the country, and the industry is huge. However, due to the large number of chromosomes (2N = 146) and the high sequence of the genome, it has been facing the problem of aggressive assembly difficulties for a long time.

The research team used the third-generation sequencing technology to combine the Bionano optical map and the HI-C high-throughput chromosome construct capture technology for the whole genome sequencing and assembly of the Chinese velvet crab in the Yangtze River Water system, and obtained a fine genome map of the chromosome level. The assembled Chinese crab genome is 1.67GB, covering 94.4%(1.77GB) of its genome size, of which the Scaffold N50 is 16.98MB and the contig N50 is 717.3KB, which identified 20,286 protein coding genes (Figure 1).

Figure 1. Genome characteristics of Chinese velvet crab chromosome level

The first secret to awaken the vitality Innexin2 gene

The word "regeneration" sounds science fiction, but in fact people's body is "regenerating" every time, such as wound repair, liver regeneration, new hair and nails ... scientists explain that compared to mammals, compared to mammals The "regeneration" ability of Chinese velvet crab is even more amazing.

For example, when the river crabs are stimulated by the outside world, the muscles attached to the base and the bottom section will shrink strongly, the broken surface is disconnected, and the "broken hands and feet" can be renewed. "This type of broken limb phenomenon is more common in production and breeding. The fracture crabs are not used for breeding and production. Most of the crabs of the broken limbs have entered the general market. The price is low. Caused a great impact, "the researchers said.

Exploration of the unique fracture of Chinese velvet crabs, with basic research and industrial application value. Wang Chenghui introduced: "We conducted a comparative genome analysis on the basis of obtaining the horizontal genome of Chinese velvet crab chromosomes to identify the unique genes and gene families unique to limb animals."

The research team expressed analysis of the comparative transcription group and genetic comparison during the different development period of the broken limb regeneration, and found that the Innexin gene family played an important molecular signal conduction role in the early days of the Chinese velvet crab broken limb regeneration. For the first time, the study was discovered that the Innexin2 gene was adjusted 1 day after the broken limb, and by regulating the MTORC1 signaling pathway and immune response response, it mediated the broken limb regeneration process of Chinese crabs (Figure 2).

Figure 2. Innexin2 regulating molecular response mechanism for Chinese velvet crab is broken limbs

Try to interpret the "compressed package" of cell differentiation

The study also discovered the early regulation of Chinese velvet crab broken limb regeneration. Among them, the SMYDA gene family only exists in the limb animals, which is expressed in the early period of the Chinese velvet crab broken limbs and expressed the response to the level when the limb buds grow to the unblocking limb. Researchers have found that the genetic family is still expressed in the overall difference in the perverts of Chinese velvet crab from large -eyed larvae to larvae, indicating that the SMYDA gene family, which is particularly special in limb animals Playing an important epigenetic role in perverted and regenerative biology (Figure 3).

Wang Chenghui explained that "SMYDA gene family is like a" compressed package 'administrator who master cell differentiation and proliferation, and important documents for cell differentiation and proliferation in the "compression package'. Kaifeng seal exposes the genetic site, causing the expression of follow -up related genes. "

Figure 3. Specific SMYDA gene family played the role of speaking genetic regulation during the regeneration of Chinese velvet crabs

The team also analyzed a comparative analysis of broken limb transcription groups with southern whitening shrimp, Rochena shrimp, and Japanese mumper shrimp. It was found that the related genes of the Innexin and SMYDA gene families have the same expression mode after the broken limb. It is speculated that the metamorphosis mechanism of the early molecular response mechanisms different from vertebrates that are different from vertebrates.

It is reported that the research provides important genome resources and platforms for the molecular breeding of Chinese crab crabs, and provides useful guidance for improving the level of breeding production and management.

---

Author: Wu Jinjiao

Edit: Chu Shuting

Editor in charge: Fan Liping

---

- END -