How trouble is the detection of nucleic acid?I'm sorry after reading

Many people think

Nucleic acid detection is like blood routine

Specimen

▽

Put on the instrument to detect

▽

Hurry up quickly

The result was found-

what is the problem?

Professionals finally answered--

Real nucleic acid detection

Not as simple as you think!

01

Sample to the specified place

Nuclear sampling is usually performed at the designated location.

02

Uniformly send the specimen to

Specific laboratory testing

Generally, the sampling point and the detection point are not together, and it is impossible to pick one, so it will be delayed here.

So some people say, can you not detect at the sampling point?

Of course not!

Nucleic acid detection is extremely sensitive and precise experiments. The nucleic acid detection laboratory has special environmental requirements, which can not be detected by just opening a place.

It is also the reason why a special square laboratory can be established in order to perform nucleic acid testing.

03

After signing the specimen

Disassembled into the system one by one

Under the close packaging, the specimen was escorted to the laboratory and was signed by the laboratory to enter the information system.

This step requires one by one for disassembly packaging, and it is a double -layer sealed packaging, and it can be dismantled for a day ...

Each one is sprayed to do a good job of biological safety protection.

With the sound of "drop", the information of the specimen will be entered into the system and connected to the health code.

During this period, all handmade, not much faster than the sampling,

Especially in massive specimens, 0 errors must be guaranteed. You cannot Zhang Guan Li Dai, or the record information is recorded, and you have to find less or not. You have to find it again.

After all, it is related to everyone's health, and carelessness is absolutely not allowed.

04

Extract nucleic acid

Test the virus like "planting peanuts"

Can't the specimen go to the laboratory, can you detect it directly?

no!

It is also necessary to proceed before the specimen and extract the nucleic acid in the specimen to expand.

Then the question is, what kind of ghost is the amplification?

If the nucleic acid extracted from the specimen is used as a piece of land, and the new crown virus is compared to peanuts, how can you know if there are peanuts in the many elements of the land?

Then the whole point of fertilizer and water, let peanuts grow, one change, two changes, four, four changes to eight ... This is easy to know.

In the same way, the virus is invisible, and the trace specimens are not easy to detect, so it is easy to make it more than to detect it.

In this process, the medical staff still had to unscrew the lid one by one and use a sample gun to draw the specimen for nucleic acid extraction —

Sit in front of the biological safety cabinet, unscrew thousands of lids, poke thousands of gun heads to draw thousands of specimens, and then kill thousands of guns, screw thousands of lids ....

For them, this step can be said to be very sour ~

05

In a completely 0 pollution environment

Preparation reagent

This step is completely 0 pollution, strict and accurate operation,

There are many reagents, how many EP test tubes, and the workload of thousands of guns ~

And all handmade packing, it must be protected, it must not be polluted

In case you fall down, you will get a wrong result:

There was no peanut in the soil, but a bunch of peanuts grew. After all, the power of the exponential increase was terrible.

06

Add samples fast, accurate, and stable

There are 0 mistakes!

This step is still all handmade, and the medical staff needs to focus on the whole process.

Make sure 0 mistakes!

And the extraction nucleic acid is easy to be polluted and easy to degrade, so the operation must be more cautious and cautious! After all, there are dozens of specimens in one board!

At the same time, the extraction nucleic acid only needs to add 5ul into the reagent system for amplification.

What is the concept of 5ul?

One -tenth of a drop of water, you can only see the point on the head with a very small gun ~ And also require the operation process: fast! Stable! Quasible!

07

On -board detection

If you start ", you can't stop

This process takes 1-2 hours, and once the instrument starts the amplification process, it cannot stop adding a new specimen midway. After the results of this batch of results are completed, the next batch of specimens can be enlarged.

Therefore, this is one of the reasons why nucleic acid detection cannot be performed at the time of testing, because as soon as the instrument is turned on, it cannot be stopped, and this batch must be run to perform the next batch.

If you really want to do it with you, then you need to say it like Akimid--

"Give me a long enough pole and a fulcrum, I can leverage the earth." Same,

Also known as--

Give me enough people, enough instruments and venues, and I can do the new crown nucleic acid specimen to go to the test.

But is it enough to finish the above?

Definitely not!

When performing each test, you also need to add the corresponding negative control, positive control, and quality control. The physiological saline control is used to monitor the quality of the entire experiment to ensure the reliability and accuracy of the test results.

After all, there are too many steps in the experimental process, and there are too many potential interference factors, so you need to take such measures per batch to ensure the quality of the test.

After the results come out, you need to check the results, check the specimen information, and release the results, and data transmission (that is, the green code).

What are the most afraid of inspectors?

It is the failure ~

Although careful and careful, it is difficult to ensure that each result is so perfect.

Then, you know ~ review the process, analyze the problem, find the steps that may be affected, and come again ~

When running instrument

Can the testing staff be idle?

Think too beautiful!

When the instrument is running, the new batch of specimens come again, and the information will be recorded, the acidity is made, and the sample ...

can not stop at all!

Many preparations are interspersed in the middle:

The consumption of the gun head (2/specimen, thousands of specimens ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ ≈ 插 插 插, only 96 gun heads ~), consumable preparation, instrument and equipment maintenance, environmental monitoring, massive garbage cleansing, massage, massive waste, cleaning, massage of garbage, cleaning, massage of massive garbage. Laboratory ultraviolet, in addition, you have to go to the toilet within one day ...

Don't worry, for your green code

Everyone is "desperate"

However, although the process is cumbersome and there are many factors, please rest assured that the medical staff have undergone rigorous theoretical operation training and assessment, and they are all holding a certificate ~

Although it is difficult to do with measuring, the results are as fast as routine blood urine, but they are also rushing to work non -stop and strictly produce results within the prescribed time.

At present, all staff members have canceled their rest and fully support the detection of nucleic acids to shorten the results of the results. Your anxiety is also their hearts.

In this way, every day, everyone cooperates to ensure that the test results of a batch of nucleic acid tests are issued on time under the super logical arrangement.

At present, the husband is responsible.

We have seen that many benevolent people are silently contributed their own strength to fighting the epidemic. Staff in various communities; whether it is a police officer or every ordinary person who actively cooperates with the government's call, let us work together to resist, and the time is difficult!

Source: Science Popularization China

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