Itching can be divided?Obilus leaf cortex found itching specific neurons

On the hot summer, mosquito bites cause itching and unbearable. Itching is a unpleasant feeling that causes people to scratch the desire for desire. Similar homicide feelings still have pain. Although itching and pain are two different feelings, it has always been closely linked to itching. In the idioms, there are many idioms of the two, such as "itching", "irrelevant pain", "not pain, no itch" Wait.

In the field of neural physiology, the neural regulation relationship between pain and itching has not been concluded, and even whether the pain and itching belong to the same feeling have not reached a wide consensus. Sucitigated theory such as Specification theory, intensity theory, selectivity theory, pattern theory, etc.

On July 29, 2022, Li Hongli, Sui Jianfeng, and Wu Guangyan of the Basic Medical College of Army Military Medical University published on the Science Advances entitled iTCH-SPECIFIC Neurons in the Ventrolalate Orbital Cortively Moduities. There is a specific neurons in selectively regulating itching in the cortex (VLO).

1. Selective markings or pain neurons through TET-OFF technology

The researchers first verified the effectiveness of the TET-OFF technology marking itching itch-the effectiveness of the neurons was verified by setting up a series of comparison experiments.

Second, the specificity of neurons in the optical fiber record, optical/chemical genetic verification mark

On the basis of marking pain or itching special neurons, researchers first observed through body optical fiber records that when large animals are injected with itching agent (5-HT, Compound 48/80, chlorine), only itching specialty sex The intensity of the calcium signal of neurons is increased, and the group of neuron calcium signals have not changed when rats are injected with pain. Similar specificity is active, and it is also observed at VLO pain specific neurons.

Further, the impact of the two types of neuron analysis on animal itching/painful behavior through optical genetic and chemical genetic technology. When inhibiting itching neurons, it significantly affects the itching of animals, but does not affect pain. In addition, when the itching neurons are directly excited, it only enhances the itching of animals without changing the pain. This result was also discovered at the regulating pain neurons. The results of these behavior experiments reveal that itching and pain may be regulated by two groups of neurons in the VLO brain area.

Third, TET-OFF markings combined with C-FOS immunofluorescence staining determine VLO brain pain, itchy specific neurons do not co-marked

Based on the results of the above behavioral experiment, the researchers further dyed the C-FOS immunochemical fluorescent staining, and achieved distinguishing the itching neurons and pain neurons on the same animals. The space is not bidding, and the itching and pain of animals are regulated by two different groups of neurons in the VLO brain area.

Summarize

The study studied the two groups of different neurons in the orbital leaf cortex at the brain level through TET-OFF technology with calcium imaging, optical genetic and chemical genetic combination. Although the nerve regulation mechanism between the outer peripheral level and the brain level itching and the pain is not completely clear, the TET-OFF mark method still provides a feasible path for the two groups of neurons with similar functions in the future.

Jiangshan and Wang Yisong of the Army Military Medical University are the first authors of the paper; Professor Li Hongli, Professor Sui Jianfeng, and Dr. Wu Guangyan of the Army Military Medical University, and Dr. Wu Guangyan are the common communication authors of the paper.

Original link:

http://doi.org/10.1126/sciadv.abn4408

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