A sensitive calcification defensive skin micro -calcification and staining method is found

The calcifying defense research team led by Professor Zhang Xiaoliang, Southeast University, published a research article of "FLUO-3AM: a sensitive calcification defense skin micro-calcification method" in Experimental Dermatology, which has attracted widespread attention.

Calculent defense is a kind of blood vessel calcification syndrome that seriously threatens life and health. The condition is dangerous and the disability and death rate is high. The disease is more common in patients with chronic kidney disease (CKD) who receive dialysis treatment. It is clinically characterized by continuous pain, ulcerative or necrotic skin damage in multiple parts of the whole body.

my country's CKD crowd is huge, and there are not a few potential calcification defense patients. In -depth early diagnosis of calcification defense is the basis and important breakthroughs of patients to improve the prognosis of patients.

The "China -University Hospital calcification defense research" team led by Professor Zhang Xiaoliang of Southeast University has long been committed to the study of diagnosis and treatment of calcification defense for a long time, especially focused on the study of early dermatology diagnosis methods of the disease.

Studies have shown that the early blood vessels have micro -calcification, interstitial, and small sweat glands in vascular calcification, and these characteristic micro -calcification can help early diagnosis. Traditional tissue dyeing methods such as HE, Alizarin S, Von Kossa can show thick calcification in the skin blood vessels, but it is difficult to identify the above -mentioned early micro -calcification, which also causes the traditional dyeing method to only be diagnosed with 56%of the calcified defense cases. Early diagnosis of calcification is facing great challenges, and more sensitive calcification detection methods are needed. Based on this prominent clinical problem, Professor Zhang Xiaoliang of the Institute of Naval Hospital of Southeast University's affiliated to the University of Southeast University recently conducted detailed research on micro -calcification testing methods. Studies have found that calcium-sensitive fluorescent dye FLUO-3AM can be used as a fast, sensitive and reliable skin micro-calcification and staining method. The preliminary results of the research have recently been published in Experimental Dermatology.

Fluo-3AM was previously used for detection of streaming cells and co-focusing microscope calcium signals. Professor Zhang Xiaoliang's research team first tried to apply FLUO-3AM to the detection of micro-calcification of skin vascular. After optimized experiments, research found that FLUO-3AM dyeing can clearly show the internal and inter-quality micro-calcification of the skin and vascular, especially for small sweat glands micro-calcification test also have high sensitivity and clarity, which will help early in the calcification defense early stage Diagnosis and reduction of missed diagnosis. Another advantage of FLUO-3 AM is the high contrast signal, and the fluorescent strength can be used for skin calcification quantitative comparison research. Fluo-3 AM can be used as an important supplement to traditional histological dyeing methods, which helps clear diagnosis of early defense. (Edit Wang Qian Editor Cheng Shouqin)

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