Where did your nucleic acid sample go?Follow Xiaojin to enter Qingyang District nucleic acid detection base to visit

For many people, the detection of nucleic acid seems to have only two steps. Registering sampling and waiting for the results. It seems that for a few hours, there is a set of rigorous and meticulous processes behind it. How is the nucleic acid sample detected? Go, follow Xiaojin to go around the Qingyang District nucleic acid detection base, which has just been "on -the -job", and take you to understand what kind of "journey" will go through the "throat" to generate report.

Entering "PCR" is just the beginning

At the Health Service Center of Wenjia Community, Qingyang District, there is a new three -story building. It is a nucleic acid detection base that Qingyang District has recently invested in. There are no special signs outside the building, only three letters of "PCR" are marked, known as the "PCR Lab", also known as the "Genetric Laboratory Lab".

"PCR" is the abbreviation of polymerase chain reaction. It is a molecular biological technology. By amplifying specific DNA fragments, it quickly grasps the patient's virus content, and its accuracy is as high as Nanomal.

Don't look at the quiet outside of the building, internal staff and machines are maintaining a high -strength operation of human -machine high -strength 24 hours.

At 7 am on July 20th, Qingyang District launched a new round of full -member nucleic acids. At nearly 400 testing points in the region, citizens lined up in an orderly manner to do nucleic acids. One hour later, the first sample was delivered to the base. At 8 am, the weather was slightly cool, and a car stopped at the main entrance of the PCR building. A commissioner wearing a protective clothing opened the trunk of the vehicle, picked up two sealing box containing nucleic acid sampling tubes, and quickly ran to the "Sample Receive Hall". Registration, verification, signing confirmation ... After everything is ready, these nucleic acid samples are sent to the unidirectional opening nucleic acid specimen passed window and started to enter a new process.

Since the launch of the nanothalette testing of Qingyang District, this newly used detection base has undertaken the important task of testing the sample testing of this round of epidemic.

A one -time nucleic acid extraction takes 90 minutes

Li Junwei is the director of the Inspection Department of the First Orthopedic Hospital of Chengdu. After the completion of the nucleic acid detection base of Qingyang District, he was transferred to the management operation of the base. Under his leadership, the reporter visited the mysterious PCR detection laboratory.

"All samples are tightly packaged in sealing bags, seals, and sealing boxes. After disinfection, they will be transported by a special car. After receiving personnel registration, checking, information entry, and disinfection, they can enter the testing link." Li Junwei Said that safety receiving samples are a critical first step. Only by ensuring that the specimen is correct can we quickly and accurately realize the traceability and review of samples.

The sampling tube after disassembling the packaging is put into the biological safety cabinet neatly and sent into the specimen extraction room. This process takes about 1 hour. In the specimen extraction room, the automatic opening and sample system will be automatically opened, the sample tube is opened, the sample is extracted, and then added to the extract reagent, then puts in the extraction RNA, and then sent to the amplification zone for amplification detection.

"There are 12 sets of such open lids and sample systems. Each set can be added to 96 sampling tubes at a time, and it takes 1 hour and 30 minutes to complete a nucleic acid extraction." According to Li Junwei, the automatic covering system can be exempted to the maximum extent. Go to artificial operation errors and risk of infection. If the leakage occurs, the system will automatically alarm to remind.

2 hours amplification "病 病" virus

In the amplification analysis room, the nucleic acid sample will go through the "long" 2 hours. The 50 expansion analyzers here will determine that your sample is "yin" or "yang".

"The samples extracted from nucleic acid sampling are trace, which is not enough to be tested." Li Junwei explained that the expansion detection uses the specified fluorescent quantitative PCR instrument, which is the size of the sample CT value obtained by the instrument detection. Whether the sample contains a new type of coronary virus. To put it simply, this process is to allow the virus to breed more in the favorite environment, so that it can be discovered in a small amount of samples.

After the amplification reaction, the technician viewed the amplification curve through the computer, analyzed the expansion results, and then judged whether the sample was positive. After the results of the instrument come out, the testing team led by Li Junwei will analyze and review the abnormal report again, and finally upload the data to Tianfu Health. At this point, a qualified nucleic acid test report is completed.

If the positive sample is found, the base will notify the inspection unit, the CDC, and the Health and Health Bureau for the first time, and immediately carry out streaming and crowd isolation. In order to prevent possible pollution, the relevant samples will be placed in a "high -pressure sterilization cooker" for sterilization after the test is over, and finally it will become an ordinary medical waste to enter the next step.

Go all out to ensure the results as soon as possible

"The busiest of the entire base is after 11 o'clock in the evening." Because most people choose to run to the place near home after get off work to do nucleic acid, many times, the sample delivery time is at 9 or 10 pm, and the test is detected to detect the test. At the time of the completion, it is usually at four or five in the morning.

“我们知道大家都希望早点查询到核酸报告，我们快一点，大家就能早一点看到检测结果。”李俊伟说，目前基地的工作人员采取三班倒，虽然许多步骤已由机器代替，但一些The connection link must be artificially intervened. Moving, signing, entry, reagent preparation, number, additional extraction, on -board expansion analysis and review ... Wearing thick protective clothing, the staff consumes great physical strength. When there are many samples sent to, in order to avoid the backlog of the sample, the staff has worked for nearly 20 hours in a row. Moreover, the entire PCR laboratory needs to be in a negative pressure environment. At the same time, the adjacent regional pressure is different to ensure that the air is clean and the pollution is avoided. Such a working environment and pressure are undoubtedly huge for the test of staff. "From the specimen of the first link to the final result interpretation, it is interlocked, and every step is sloppy. Our most important task is to accelerate and sieve the positive cases!" The staff said that in the laboratory, what is needed is the sense of responsibility, carefully and patiently detecting each sample. "Our results and judgments will determine whether a person will be isolated, and whether a building or a community is closed, of course, be careful."

The reporter learned that in order to maintain the future efficient operation of the base, Qingyang District will select a group of medical staff to carry out rotation training to cultivate a batch of backbones. The nucleic acid testing team of the battle "to better meet the needs of the nucleic acid testing of Qingyang citizens.

Chengdu Daily Jinguan News Zhang Jing reporter Chen Yong Picture Qingyang Rong Media provides responsible editor He Qixie Internship Editor Lu Yarui

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