Wu Fei, a student of the School of Life Sciences, Anhui Agricultural University

■ Struggler is youth

Wu Fei, a student of the 2018 Biological Innovation Experimental Class of the School of Life Sciences, Anhui Agricultural University, has just won the 17th "Challenge Cup" National College Extracurricular Academic Science and Technology Works Competition for the 17th "Challenge Cup".

Although the name of the project is obscure and difficult to understand- "The" Penicillin "of the prevention and treatment of plant diseases -the Study on the New Special Microbial fungicide of termites symbiotic bacteria", Wu Fei said that it was the Tao.

"Plant diseases have a direct economic loss of hundreds of billions of dollars each year, of which 70%to 80%are caused by plant fungal diseases. And due to the long -term dependence of chemical pesticide prevention and treatment of plant fungal diseases, it brings serious food safety, and it brings severe food safety. Environmental ecology and drug resistance. "As a student in biological majors, Wu Fei learned the dilemma in the field of discipline very early.

"Can you develop better pesticide preparations to reduce the losses caused by plant fungal diseases?" With doubt, Wu Fei and other students found Zhang Yinglai, a teacher of the School of Life Sciences.

In recent years, the advantages of biological pesticides due to their good prevention and control effects, fast decomposition under natural conditions, and less residues after use, and have received attention from people. Under the guidance of Zhang Yingzheng, Wu Fei turned his eyes to special microbial resources.

"Just like the farmers' crops will be harmed by pathogenic bacteria, and bacteria -breed worm termite will also be harmed by pathogenic bacteria, but they can use the secondary metabolic products produced by their symbiosis . This has given us inspiration, can we screen the target strains from the symbiosis and make the bacteria insect symbiosis a new source of microbial pesticides? "Wu Fei said.

After a lot of research and practice, black -winged terphlosy symbiosis eventually became the team experiment.

During the university, after thousands of kilometers of trek, the team went to Anhui, Jiangsu, Zhejiang and other places to collect samples to conduct a comprehensive anatomy of black -winged and termites, intestinal tracts, and ant nests. The secondary metabolic products are tracked and separated, and finally improved the output through the cultivation conditions optimization and metabolic engineering. The breeding library saves 120 strains with excellent traits, and 27 antibacterial activated substances are separated from them.

"For the first time, we obtained the strains of high -yield rhinopine methar ether from the termite symbiotic bacteria, and the output reached 28.0 mg/L. This is the most recorded in the literature. The production cycle of plant source rhiline methar ether has a long production cycle and high cost, which provides a new type of preparation for high -active plant source agricultural sterilizers with independent intellectual property rights in my country. "Speaking of which, Wu Fei is full of pride.

The team not only develops agricultural antibiotics with high value for science and technology such as rhubarin methar ether, but also pays attention to the research of living microbial preparations. "In practice, we have obtained a very effective application effect from the termite nest, which has a significant prevention of plant fungal disease and has a good market application prospect."

Today, Wu Fei and her team have applied for the national invention patent for the achievements of the two items of industrialized application. It only took 10 months from the application to authorization.

Wu Fei said that the team will be committed to strengthening new products for biological pesticides with special microbial resources to strengthen the development of green agriculture. (Reporter Fang Mengyu Correspondent Xia Liming)

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