Fundamental Research reports new progress in our school's fish immunology research

Recently, Professor Liu Qin, National Key Laboratory of the School of Biological Engineering and Biological Reactor Engineering of our school, and the team of Associate Professor Yang Dahai have built the richest fish immune cell maps so far. Cell LandScape of Turbot (ScophThalmus Maximus "was published in the National Natural Science Foundation Fundal Research magazine.

Dalingyu (commonly known as "Duobao Fish") is an important seawater breeding fish species in my country. It adopts the "deep well greenhouse" breeding model. Its breeding system is stable and the formula feed is perfect. It is a model of my country's factories. In recent years, the research on the survey of Dalingju in my country has been clear, infection and molecular immunology research has also made breakthroughs in the development of immunological products such as vaccines. Many of the above factors make Dalingyu the first choice model for the practice of comprehensive immune prevention and control of aquatic diseases. However, in general, the foundation of Dalingbu infection and cellular immunology is still relatively weak, which obviously restricts the rational design and application evaluation of immune biological products such as vaccine, immune enhancers, and micro -ecological preparations. Effective advancement of immune prevention and control strategy.

In recent years, the research of infection immunology of major aquaculture fish species in my country has achieved more results at the level of infection and molecular immunology, but the research at the level of cell immunology has been slower for a long time. Because immune cells have high heterogeneity and in dynamic response changes, how to effectively analyze the characteristics of changes in different quality elements in complex networks is an important challenge we face.

The above industrial demand is guided. The research group is targeted at Dalingyu. Based on the ISO-SEQ technology, the Datong-鲆 transcription group database is improved, and the structure and function comments quality of the Dalingji gene (Marine Genomics 2022), and in On this basis, the most rich fish immunocyte map has been established through the sequencing technology of the single -cell transcription group: successful identification of macrophages, neutral granules, dendritic cells, natural killing cells, and two inherent inherents. Lymphocytes (ILCS3-1-Like and ILCS3-2-Like), clarifying ten types of T/B cells (including IGM+IGD+B cells, IGM+IgD+pulp cells, IGT+B cells, CD4+childish T cells, CD4+ FOXP3+ and CD4+ Foxp3-regulatory T cells, TH17 cells, CD8+ childish T cells, CD8+ cytotoxic T cells, and CD4-CD8-T cells), and analyzed the potential logo molecules of the above-mentioned fish immune cells for targeted analysis The function of related immune cells provides public resources.

At the same time, this article also analyzes the dynamic changes in the dynamic changes of the primary infection of the infected cells in the cell in the infected cells from different time points of different immune organs and pathogenic infections at different time points. Studies have found that type I interferon may play an important role in the early response of congenital immune cells. It clarifies that fish B lymphocytes may play an immune defense function in the early stage of bacterial infection, clarifying CD4-CD8-T cells, CD8+cytotoxic T cells , TH17 cells and their mirror cells ILCS3-2-Like have cytotoxic and regulatory dual functional characteristics in the process of bacterial infection, which lays the theoretical foundation for in-depth analysis of the adaptive immune cell function of hard bone fish.

In summary, the article analyzes the mechanism of action of fish immunomies, cells and molecules in the process of anti -infective from the perspective of infection immunology, and clarifies the composition and dynamic response of the inherent immune and adaptive immune system of fish. Research results are of great significance to the design and application of the design and application of aquaculture -breed economy fish and the design and application of aquatic animal disease prevention and control strategies.

Chen Weijie, Wang Wei, Wang Ying, and Huang Jianzheng, a doctoral student in our school, were the first authors of the dissertation, Professor Liu Qin and Associate Professor Yang Dahai as the communication authors of the dissertation. During the completion of the paper, Professor Zhang Yuanxing and Professor Wang Qiyao were strongly supported. The study was supported by the National Natural Science Foundation of China and outstanding youth funds.

Original link: https://www.sciencedirect.com/science/article/pii/s2667325822000668

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