The Hua Nong team made the rape gene reorganization and mutation "there is a spectrum"

The Yangtze River Daily Da Wuhan Client September 6 (Reporter Zhou Yan Correspondent Jiang Chao Chang) Recently, the rapeseed genetic improvement team of the Central Agricultural University of China released a variety of rapeseed reorganization, exploring the full genome reorganization between the two main genetic groups of rapeseed Features, it provides important clues for the genetic reorganization of different rapeseeds, which has important value for genetic diversity, crop improvement, and genome assembly research.

Related research papers are published online in the latest issue of the important English academic journal hosted by China. The first author is Wang Meng, a doctoral student of Huazhong Agricultural University, and the corresponding author of the school is Professor Zou Yan of the school's rape breeding resource innovation project group. This is also the latest achievements in the genome structure variation and genetic evolution of genomic structure of rapeseed resources in the breeding resource innovation of rapeseed resources in Huazhong Agricultural University to carry out rapeseed species, inter -seed hybridization and germination of offspring, genetic analysis of the complexity of rapeseed, and the selection of whole genome one.

Yesterday, a reporter from the Yangtze River Daily interviewed Wang Meng, the first author of the article. According to its introduction, the most widely planted rapeseed types are cabbage rapeseed, which is doubled from the natural hybridization of cabbage and cabbage. It is a young species in the main oil crops in the world. Cabbage (AA, 2N = 20) and cabbage (CC, 2N = 18) are duplex species, while cabbage rapeseed (AACC, 2N = 38) is four times heterogeneous, and the genome is relatively complicated. In the current cabbage -type rapeseed, the genetic distance between European winter rapeseed and Chinese semi -winter rapeseed is relatively far away, and there are differences in their respective properties and differences. It can be hybridized. Features of genetic reorganization.

Seemingly a simple map, in fact, contains long patient waiting. Wang Meng started to contact the direction of the project from entering the laboratory. Until now, the result was only seen in seven or eight years. The reason is that we must understand both parents and children. After more than ten years of hard work, the Chinese team and several other teams in the world have completed the whole genome sequence of Tapidor in European winter rapeseed. The Chinese team (Huazhong Agricultural University and Zhejiang University) also sequensed China half of the winter half winter. Sexual rapedine oil No. 7. At the same time, it has also completed the sequencing work for the genealogy of these two pro -based materials. With the information of these two physical maps and breeding scores, the genetic information of the parents is finger; then, the planting, trait inspection, and genetic types of hybrid descendants have been completed; in the end, the China Central Agricultural University team has built them. The reorganization of the hybrid offspring group analyzes this map. The reorganization has occurred in which places have occurred, and there are no reorganizations.

The TN group marking and its restructuring rate variation distribution on the Chinese semi -winter rapeseed NY7 genome.

The interesting conclusion comes from a detailed comparison. The research team evaluated the reorganization of the whole genome and found that 93%of the genome can detect the reorganization exchange. That is to say, it will be affected by the parents. On the contrary, it is basically not affected. In 93%of the genome, there are 121 places that are particularly prone to reorganization, that is, reorganization hotspots (70 in the A genome), in the 7%genome, there are 16 places that are not easy to reorganize, that is, the cold spots (reorganization (reorganization (cold spots ( 12 in C genome). There are differences in the level of genetic reorganization of descendants with different pro -proof backgrounds. The descendants of the descendants with the background of Chinese half -winter rapeseed oil 7 are higher than the descendants of the heredity background of Tapidor in Europe. This may also be affected by the growth environment. The influence may also be related to the penetration of cabbage breeding during the process of breeding.

The genetic structure of a single DH series and its two-way return to offspring is distributed with Identity-By-Descent (IBD).

The reorganization mutant map will also be a "treasure map" that assisted artificial breeding. Knowing the genetic reorganization hotspots and cold spots will be conducive to accurate breeding with molecular technology. It contains the genetic sites that control the oil content of the seeds. The area where these sites are located are easy to reorganize, so people are easy to complete the rapid accumulation of control seed oil content favorable genes in this area, which helps the breeding of high oil content rapeseed. In addition, the offspring created in this study also as a resource for gene cloning. These results and rich genome resources can broaden the understanding of heterosexual multi -twice reorganization behaviors, and provide reference for genetic improvement of rapeseed.

[Edit: Deng La Xiu]

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