Shanxi University has made new progress in biological catalytic

Recently, the team of Professor Yang Hengquan of the School of Chemistry and Chemical Engineering of Shanxi University has made new progress in biological catalytic. The research is based on the topic of "Co-compartmentalization of Enzymes and COFActors within PicKKERING EMULSION Droplets for Continuous Flowest. . ED.

The continuous flow of biological catalytic highlights, pure hand -alcohol, handicide and other pharmaceutical intermediates have attracted widespread attention from academic and industrial circles, and have become my country's technology and industrial development strategy. The biological catalytic response process often requires the participation of enzymes and auxiliary factor. Although the previous research proposes the fixing method of multiple enzymes and auxiliary factor, there are still problems such as the complex factor that is difficult to circulate and poor catalyst stability. Running requirements.

On the basis of continuous liquid oil-water dual-phase catalytic research in the early stage, Professor Yang Hengquan proposed a continuous flow biological catalyst system fixed in Pickering's milk drops. This system can restricted the water -soluble auxiliary factor in the milk droplet, and at the same time, enzyme adsorption is attracted to the milk drop interface, thereby realizing the common fixing of enzymes and auxiliary factor in micro -scale space. During the flow, enzymes can freely contact the auxiliary factor, and regenerate the auxiliary factor in the interior of the milk droplet by adding a co -substrate in the continuous phase. Taking the enzyme catalytic asymmetric reaction borrhane alcohol involved in the participation of the auxiliary non -degrad NADPH. 2.7 times, and remained high catalytic activity, selectivity, and TTN (59204 mol mol-1) after 350 hours of continuous response. At the same time, the system has good universality. In the enzyme catalytic asymmetric transit reactions of the auxiliary factor PLP, the system also shows high catalytic efficiency and prominent continuous operation stability (400H) , Much higher than the current industrial indicators. Studies have also found that by changing parameters such as changing the concentration of the substrate, the auxiliary factor concentration, and the drip size, the precise regulation of the catalytic efficiency of the reaction system can be achieved. The continuous flow of the Pickering emulsion realizes the efficient coupling of enzymes and auxiliary factor in time and space, and will promote continuous flowing biological catalytic development towards a practical level.

The first author of the paper is Wei Wei, a doctoral student in the school, and the corresponding author is Professor Yang Hengquan. This work was funded by projects such as the National Natural Science Foundation of China, the "1331" project and corporate project of Shanxi Province. (Correspondent: Zhang Ying, Shanxi University)

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